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Blue Myco

25Q Fully Automatic Electric Pressure Sterilizer Autoclave 110V

$700.00
Taxes, discounts and shipping calculated at checkout.

The Bonsai 25Q is a compact benchtop autoclave for growers who sterilize every week and are tired of babysitting a stovetop pressure cooker. Set your temperature, set your time, walk away. It reaches sterilization pressure in about 25 minutes on a standard 110V outlet, holds 15 to 20 PSI, and alarms when the cycle is done.

  • 25 qt~23.7 L chamber
  • 14wide-mouth quart jars
  • 110V2 kW, standard outlet
  • ~25 minto pressure
  • 38 lbships parcel, no freight

Rewired for the US before it ships

Imported benchtop autoclaves have a reputation, and the wiring is usually why. Bonsai imports these units and replaces the original wiring with heavy-duty American wire, along with several other component changes, before they go out the door.

That is the difference between this and the near-identical looking units you will find on overseas marketplaces for less. The chamber may be similar; what carries the current is not.

Why this instead of a stovetop pressure canner

A stovetop canner works, but it needs a burner, constant attention, and manual pressure management on every load. The 25Q is a sealed electric unit with its own heating element and digital control. The difference is not sterilization quality — both reach 121°C — it is what you do during the cycle.

Bonsai 25Q Stovetop canner
Heat source Built-in 2 kW element, 110V outlet Your stove or a propane burner
Pressure control Automatic, digital setpoint Manual — you watch the gauge
Attention required Load, set, walk away Monitored for the whole cycle
Cycle end Auto shut-off plus audible alarm You have to be there
Stove tied up No Yes, for hours

What fits inside, and how long it takes

The chamber is tall and narrow rather than wide, which is what lets a 25-quart unit take a full rack of quart jars standing upright.

Load Capacity per cycle Typical hold time
Wide-mouth quart jars (grain) Up to 14 90 min at 121°C
Liquid culture jars Up to 14 25–30 min at 121°C
Agar media flasks Varies by vessel 25–30 min at 121°C
Tools and utensils Loose in basket 25–30 min at 121°C

Add roughly 25 minutes to reach pressure, plus cool-down, on top of the hold times above. Small-batch spawn bags work too, provided they sit loosely enough for steam to circulate.

Running a cycle

  • Remove the inner stainless basket and disconnect the lid tubing.
  • Place the aluminum grate in the bottom of the chamber.
  • Add purified water, enough to cover the heating elements — roughly 1 cm below the bottom tray.
  • Load your jars, bags, media, or tools.
  • Close the lid and tighten the hand wheel knob.
  • Set the temperature to 121°C (250°F) and enter your hold time.
  • Start. The timer begins once the chamber reaches setpoint, around 15 to 17 PSI.
  • Wait for the completion alarm, then vent and unload.

Water matters. Use purified or RO water. Hard tap water will scale the heating element over time and shorten its life. This is the single most common maintenance mistake we see on these units.

Specifications

Chamber capacity 25 quarts (approx. 23.7 L)
Jar capacity Up to 14 wide-mouth quart jars
Construction 100% stainless steel
Heating element 2 kW
Electrical 110V standard household outlet
Working pressure 15 – 20 PSI
Temperature range 105°C – 126°C (221°F – 259°F)
Time to pressure Approx. 25 minutes
Controls LED panel — set temperature and hold time
Lid Quick-latch hand wheel
Included Removable stainless basket, aluminum bottom grate
Drainage Water drain outlet
Shipping weight 38 lbs — ships parcel, no freight
Warranty 1 year

Safety

  • Double safety valve system — redundant over-pressure protection
  • Automatic shut-off — the unit powers down the element at cycle end
  • Completion alarm — audible signal so a finished cycle is never left sitting
  • Quick-latch hand wheel — positive lid engagement before pressure builds

The only unit here that needs no electrical work

Every other sterilizer we sell runs on 220 or 240 volts, which means an electrician, a dedicated circuit, and a freight delivery before you sterilize anything. The 25Q plugs into the wall and ships by parcel carrier at 38 lbs.

Give it its own circuit if you can. Nothing special is required, but a 2 kW draw sharing a kitchen circuit can trip a breaker mid-cycle.

Sterilization only — this model does not pasteurize

The 25Q runs from 105°C upward and does not reach the low temperatures a true pasteurization cycle requires. For substrate you intend to pasteurize rather than sterilize, you need a commercial chamber that holds a lower setpoint — our B-310L and larger models run both processes.

Common questions

Is the Bonsai 25Q a true autoclave or just a pressure cooker?

It is a true steam autoclave. It reaches and holds 121°C at 15 to 20 PSI with automatic digital pressure and temperature control, which is the standard sterilization condition for laboratory media. The difference from a pressure cooker is the control system and the automatic shut-off, not the sterilization itself.

How many jars fit in the 25Q?

Up to 14 wide-mouth quart jars standing upright. The chamber is tall and narrow rather than wide, which is what makes that count possible in a 25-quart unit.

Does it need a special electrical outlet?

No. It runs on a standard 110V household outlet and draws 2 kW. No 220V service, no electrician, no dedicated circuit installation. Give it its own circuit if you can, simply because a 2 kW draw on a shared kitchen circuit can trip a breaker.

How long is a full grain jar cycle?

Plan on 90 minutes of hold time at 121°C for grain, plus roughly 25 minutes to reach pressure and cool-down afterward. Liquid culture and agar media need only 25 to 30 minutes of hold time.

Can it sterilize spawn bags as well as jars?

Yes, for small-batch work. Bags need to sit so steam can circulate rather than being packed tight. If you are running large volumes of bags every week, a floor-standing unit like the 150L will suit you better than a benchtop machine.

How does it compare to an All American stovetop sterilizer?

An All American is an excellent piece of equipment and will outlive almost anything. The difference is workflow. The All American is a stovetop unit you monitor manually for the entire cycle; the 25Q is self-contained, reaches pressure faster, holds the setpoint automatically, and shuts itself off. If you want a machine you can load and leave, the 25Q is the one. If you want something with no electronics to ever fail, the All American is.

What maintenance does it need?

Use purified or RO water to prevent scale, keep the lid gasket clean and free of debris, and replace the gasket about once a year, or every six months under heavy daily use. Replacement gaskets are stocked in our parts collection.

Can I pasteurize substrate in it?

No. The lowest setting is 105°C, well above pasteurization range. For that you need the B-310L or larger.